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  • 第60回がん研先端研究セミナー(12月10日)のお知らせ「Untangling genomic DNA in mitosis for faithful chromosome segregation; Tale of TopoIIα tail」Yoshiaki Azuma, Ph.D (Department of Molecular Biosciences, University of Kansas)

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第60回がん研先端研究セミナー(12月10日)のお知らせ「Untangling genomic DNA in mitosis for faithful chromosome segregation; Tale of TopoIIα tail」Yoshiaki Azuma, Ph.D (Department of Molecular Biosciences, University of Kansas)

2019年12月05日

第60回がん研先端研究セミナー(12月10日)のお知らせ

 

演題:Untangling genomic DNA in mitosis for faithful chromosome segregation; Tale of TopoIIα tail

 

演者:Yoshiaki Azuma, Ph.D (Department of Molecular Biosciences, University of Kansas)

 

抄録:Chromosome segregation during mitosis is a fundamental process in which the replicated genome is evenly inherited by two daughter cells. Paired sister chromatids are formed after DNA replication and are held together by a protein known as cohesion as well as by the entanglement of genomic DNA. In comparison with the well-described resolution of cohesin-mediated sister chromatid cohesion, the regulation of DNA-entanglement resolution during mitosis has not been well-studied. Without proper regulation of these resolution mechanisms, the cell cannot maintain genomic integrity, and this could result in defective function after cell division. The key enzyme that regulates DNA-entanglement during mitosis is DNA Topoisomerase II (TopoII), which is an essential enzyme for eukaryotes. TopoII performs unique strand passage reaction (SPR) through which one double stranded DNA molecule is cleaved and then passed through another intact double stranded DNA to resolve tangled genomic DNA. Our recent study of SUMO (Small Ubiquitin-like MOdifier)-modified TopoII during mitosis provides a mechanistic insight into how eukaryotic cells solve the problem of entangled genomic DNA during mitosis. Our results suggest that SUMO-modified TopoII can control progression of mitosis by activating a mitotic checkpoint. This mechanism allows cells to have time for resolving the entangled DNA. In addition, SUMO-modified TopoII is targeted by a putative nucleosome remodeling protein which could resolve entanglement of genomic DNA mediated by defected TopoII activity. Based on our findings, we propose a novel regulatory mechanism of entangled genome via SUMOylation of TopoII on mitotic chromosomes and I would like to discuss further questions to be addressed for maintaining integrity of the genome during mitosis.

 

 

日時:2019年12月10日(火)16:00〜

 

場所:がん研究所 4階 435号室

 

連絡先:斉藤典子(内線5474)

 

*外部の研究者のご来聴を歓迎いたします。尚、本セミナーの内容は専門的であり、医学・生物分野の研究に携わる方を対象としております。

 

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