【がん研セミナー(11月29日)のお知らせ「Single molecule detection of DNA replication fork dynamics」Conrad A Nieduszynski, Ph.D.（Earlham Institute，UK）】
2022年11月01日
がん研セミナー(11月29日)のお知らせ
演題：Single molecule detection of DNA replication fork dynamics
演者：Conrad A Nieduszynski, Ph.D.（Earlham Institute，UK）
抄録：Replication of eukaryotic genomes is highly stochastic, making it difficult to determine the dynamics of individual replication forks across the genome. Single molecule genomic technologies have the potential to detect replication fork dynamics hidden in the cumulative cell view provided by traditional genomic datasets. Here we present our findings using nanopore sequencing (D-NAscent) to detect replication fork dynamics across the genome in yeast and human cells. D-NAscent detects the changes in BrdU incorporation during S phase to determine the direction of replication fork movement on individual nanopore sequence reads. This identifies the sites of replication fork initiation, pausing and termination across the genome, at near base resolution and on single molecules.
日時：２０２２年１１月２９日（火） １６：００ 開始
場所：吉田講堂とオンライン配信によるハイブリッド形式
＊新型コロナ感染拡大対策のため、吉田講堂での参加は先着順５０人を上限とします。
事前入場予約をご希望の方は前日までにtminamisawa@jfcr.or.jpまでお申し込みください。
＊オンライン参加の方は以下のURLから事前登録後、お送りするZOOMアドレスからご参加下さい。
https://zoom.us/webinar/register/WN_wHnXD5TsRmOWyIjSXXhkaA
連絡先：大學 保一
演題：Single molecule detection of DNA replication fork dynamics
演者：Conrad A Nieduszynski, Ph.D.（Earlham Institute，UK）
抄録：Replication of eukaryotic genomes is highly stochastic, making it difficult to determine the dynamics of individual replication forks across the genome. Single molecule genomic technologies have the potential to detect replication fork dynamics hidden in the cumulative cell view provided by traditional genomic datasets. Here we present our findings using nanopore sequencing (D-NAscent) to detect replication fork dynamics across the genome in yeast and human cells. D-NAscent detects the changes in BrdU incorporation during S phase to determine the direction of replication fork movement on individual nanopore sequence reads. This identifies the sites of replication fork initiation, pausing and termination across the genome, at near base resolution and on single molecules.
日時：２０２２年１１月２９日（火） １６：００ 開始
場所：吉田講堂とオンライン配信によるハイブリッド形式
＊新型コロナ感染拡大対策のため、吉田講堂での参加は先着順５０人を上限とします。
事前入場予約をご希望の方は前日までにtminamisawa@jfcr.or.jpまでお申し込みください。
＊オンライン参加の方は以下のURLから事前登録後、お送りするZOOMアドレスからご参加下さい。
https://zoom.us/webinar/register/WN_wHnXD5TsRmOWyIjSXXhkaA
連絡先：大學 保一
