第8回先端研究セミナー

演題：To what do microparticles from blood plasma look like?

　　　Their morphology, size, phenotype revealed by cryo-EM and immuno-gold labeling.

演者：Alain Brisson, Ph.D.

　　　Molecular Imaging and NanoBioTechnology – UMR-CBMN 

　　　University of Bordeaux - CNRS, France

抄録：　

 Microparticles (MPs), also called extracellular vesicles, microvesicles, attract high interest as potential disease biomarkers, however many basic questions concerning them remain unanswered, such as: To what do MPs from blood plasma look like? What is their morphology, their size distribution? Do they all bind Annexin-5 (Anx5)? How are they formed? How many are they? 

  Our overall aim is to answer these questions, firstly to improve our basic understanding on MPs, secondly to design diagnosis assays on selected MP sub-populations. Our first objective was to provide a catalogue of all types of MPs present in plasma from healthy donors, considering this as a reference for further studies of MPs in various pathological conditions. 

  We have developed an original approach based on cryo-Transmission Electron Microscopy (cryo-EM) imaging and receptor-specific gold labeling. Cryo-EM allows revealing the various types of MPs present in pure platelet free plasma (PFP). Gold nanoparticles functionalized with various ligands were developed for identifying specific MP sub-populations. We focused primarily on MPs exposing phosphatidylserine (PS), platelet-derived MPs and red blood cell-derived MPs, using gold particles functionalized with Anx5, anti-CD41 and anti-glycophorin antibodies, respectively. 

  In this lecture, I will present our current vision of MPs in blood plasma.

日時：２０１３年　２月６日（水）　１５：００〜１６：００

場所：研究所１階　セミナー室Ａ

連絡先：芝　清隆（内線5382）

＊外部の研究者のご来聴を歓迎いたします。尚、本セミナーの内容は専門的であり、医学生物学分野の研究に携わる方を対象としています。