- How chromosomes are assembled in mitosis?
- How kinetochore-microtubule attachment is controlled?
- How do cells induce chromosome segregation at the correct time?
- Mechanisms of chromosomal instability in cancer cells
How do cells induce chromosome segregation at the correct time?
Separation of sister chromatids has long been recognized as a drastic and irreversible event in the cell cycle. A fascinate feature of this process in mammalian cells is that the separation occurs in a highly synchronous manner at the given moment: all sister pairs separate at the same time. This abruptness of anaphase onset is phenomenologically well known, but we are far from understanding it at the mechanistic levels. I am very interested in understanding how do cells achieve this remarkable feat, and therefore we aim to characterize morphological and biochemical changes during the metaphase-to-anaphase transition, in high time and space resolution.