Experimental Pathology

Update : 2022/04/14


Loss or gain of chromosomes is associated with many cancer cells. This property, called chromosome instability, might arise from a lesion in the chromosome segregation machinery. In mitosis, chromatin is structurally reorganized into chromosomes in which replicated DNA molecules are seen as sister chromatids. Each chromosome is then captured by two opposing poles of the spindle which allows the segregation of sister chromatids towards opposite poles. Our ultimate goal is to understand how chromosomes condense, achieve bipolar spindle attachments and how cohesion is subsequently dissolved to allow sister chromatid separation. Elucidating these processes should provide insight into the mechanisms underlying chromosome instability in cancer cells.Original Web SiteNew Window


  1. How chromosomes are assembled in mitosis?
  2. How kinetochore-microtubule attachment is controlled?
  3. How do cells induce chromosome segregation at the correct time?
  4. Mechanisms of chromosomal instability in cancer cells

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Uchida, KSK., Jo, M., Nagasaka, K., Takahashi, M., Shindo, N., Shibata, K., Tanaka, K., Masumoto, H., Fukagawa, T., and Hirota, T.
Kinetochore stretching-mediated rapid silencing of the spindle-assembly checkpoint required for failsafe chromosome segregation.
Curr. Biol. 31: 1581-1591 (2021)
Shindo, N., Otsuki, M., Uchida, KSK., and Hirota, T.
Prolonged mitosis causes separase deregulation and chromosome nondisjunction.
Cell Rep. 34: 108652 (2021)
Matsui, S., and Nozawa, R.S.
RNA impacts formation of biomolecular condensates in the nucleus.
Biomed. Res. 42: 153-160 (2021)
Jo, M., Kusano, Y., and Hirota, T.
Unraveling pathologies underlying chromosomal instability in cancers. Review.
Cancer Sci. 112: 2975-2983 (2021)


Toru Hirota, M.D., Ph.D.
The Cancer Institute
Japanese Foundation for Cancer Research (JFCR)
Ariake 3-8-31, Koto-ku Tokyo, 135-8550
Tel. +81-3-3570-0446
E-mail :

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